DuPont™ AmberChrom™ Fine Mesh Ion Exchange Resins for Peptide and Oligonucleotide Column Separations

Daryl Gisch and Jennifer Giblin – DuPont

DuPont™ AmberChrom™ fine mesh ion exchange resins have many resin design features that impact performance, giving peptide drug developers many degrees of freedom to design purification processes that balance impurity removal with ease of operation. This poster shows the interdependence of particle size, degree of crosslinking, and ionic exchange capacity on peptide uptake and purification.

The binding and elution of large organic molecules like peptides present challenges to standard ion exchange resins due to slower kinetics in the uptake and elution processes. Fine mesh resins, on the other hand, are smaller resins that offer faster kinetics. These resins are available in a variety of options to allow end-users to choose a resin with the optimum levels of water permeability, water retention capacity, total capacity, and operational kinetics for their targeted peptide purification process. AmberChrom™ fine mesh ion exchange resins are physically durable, pH tolerant, and can used over a wide range of buffers and insoluble in common solvents. The product features enhance laboratory separations and allow easy adjustments during process scale-ups.