Comparison of Reverse Phase Resins for Preparative Oligonucleotide Separations

Martin Deetz, Nicholas Samulewicz, and Jay Dharia b DuPont

High purity nucleotide oligomers are important for therapeutic and research uses. Obtaining these high purity oligos requires purification steps to remove inpurities generated during the synthesis.

It is well known that reverse phase chromatography (RP-HPLC) is an excellent method for oligo purification, but it can be difficult to know where to start when it comes to resin selection. In this study, we demonstrate the purification of crude DNA and RNA oligos with DuPontb" AmberChromb" XT20 and compare the impact versus different resins on the resulting yield and purity.

Process development scientists can utilize these trends in helping to design scalable processes for high purity oligonucleotides.