Purification of Nucleotide Triphosphates: Critical Building Blocks for mRNA Therapies

Martin Deetz, Dorie Yontz, and Michael Ostrander - DuPont

High-purity nucleotide triphosphates are essential to ensure efficient deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) synthesis. Researchers rely on purified nucleotide triphosphates as the building blocks of messenger RNA (mRNA) technology, which is fundamental to applications such as COVID-19 vaccines. However, commercially available sources of nucleotide triphosphates can be contaminated with mono-, di-, or even tetra-phosphates. This article discusses the importance of high-purity nucleotide triphosphates and how fine mesh anion-exchange resins can be used to purify them.